Inhibitory Effect of Polyphyllin D on Proliferation of Human Colon Cancer Cell Line SW480 via the MicroRNA-21/Phosphatase and Tensin Homolog Deleted on Chromosome 10

To evaluate the effects of polyphyllin D on proliferation and apoptosis human colon cancer SW480 cell lines to reveal whether this process is mediated by micro ribonucleic acid-21/phosphatase tensin homolog deleted chromosome 10 pathway. Human cells were transfected with acid-21-mimic or mimic-negative control treated at different concentrations (control, 0.25, 0.5, 1 2 μM) for 24 h 48 h. Compared group, viability significantly declined but rate rose in 0.5 μM group colony number decreased 0.25 (p<0.05). had increased protein expressions Bcl-2 associated X cleaved caspase-3, expression B-cell lymphoma-2 (p<0.001). The levels acid-21 phosphorylated-protein kinase B lower, while phosphatase messenger acid higher than those However, was not changed (p>0.05). After transfection acid-21-mimic, level higher, lower antiproliferative pro-apoptotic reversed acid-2-mimic Polyphyllin inhibits induces via